![]() ![]() The result of this should have a flat (even) background that should appear white or neutral (unless the glass slide has a noticeable tint) as long as you did not change any of the settings of the camera between shots (time, exposure, white balance etc) or the microscope settings (light, condenser, magnification). ![]() Run ( "Calculator Plus", "i1=Numerator i2=Divisor operation= k1=255 k2=0 create" ) This is achieved in a single step with the Calculator_Plus plugin which performs the division, followed by a multiplication (and a sum if needed) all in double precision before pasting the clipped result back into an image: This number cannot be stored in an 8 bit per channel image instead the value is re-scaled to the range of 0-255 per channel by multiplying the division result by 255. The result of this division is a real number between 0 and 1 (0 if there is no light at all passing through the specimen, 1 if all the incident light passes through the specimen). That is, the division of images: Numerator / Divisor. Next, we calculate the transmittance as the ratio of transmitted light through the specimen and the incident light to produce the corrected image. ImageCalculator ( "Subtract create", "Specimen", "Darkfield" ) ![]() SelectWindow ( "Result of Brightfield" ) ĭo the same with the Specimen image: (Specimen - Darkfield) and call the result “ Numerator”. ImageCalculator ( "Subtract create", "Brightfield", "Darkfield" ) Using the command Process>ImageCalculator, calculate (Brightfield - Darkfield), and call the result “ Divisor”: The operation (in a 8 bit channel) consists of calculating the transmittance through the specimen:Ĭorrected_Image = (Specimen - Darkfield) / (Brightfield - Darkfield) * 255įirst we will compensate the electronic bias (hot pixels) in the Brightfield and Specimen images. ![]()
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |